Recombinant muis S100A4 eiwit

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 have been implicated in tumor metastasis. Recombinant mouse S100A4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

13.9 kDa (121aa) confirmed by MALDI-TOF

S100A4, S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a, ATGP1080-10 µg, ATGP1080-20 µg, ATGP1080-50 µg, ATGP1080-100 µg, ATGP1080-250 µg, ATGP1080-500 µg, ATGP1080-1 mg, ATGP1080-10, ATGP1080-20, ATGP1080-50, ATGP1080-100, ATGP1080-250, ATGP1080-500, ATGP1080-1

Sherbet GV., et al. (2009) Cancer Lett. 280 (1) :15-30.; ; Ambartsumian N., et al. (1995) Gene. 159 (1) :125-30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl.

Cancer

NP_035441

P07091

Mouse

MARPLEEALD VIVSTFHKYS GKEGDKFKLN KTELKELLTR ELPSFLGKRT DEAAFQKVMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGCPDKEPRK K