Recombinant muis S100B eiwit

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Recombinant mouse S100B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

S100 calcium binding protein B, S100 protein beta polypeptide neural, S-100 protein beta chain, S-100 protein subunit beta

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

12.8 kDa (112aa) confirmed by MALDI-TOF

S100B, Protein S100-B, AI850290, Bpb, Protein S100-B, NEF, S 100 protein beta chain, S 100 protein beta subunit, S100, S100 calcium binding protein B, S100 calcium binding protein beta (neural), S100 calcium binding protein beta chain, S100 protein beta chain, S100B, S100beta, ATGP0608-10 µg, ATGP0608-20 µg, ATGP0608-50 µg, ATGP0608-100 µg, ATGP0608-250 µg, ATGP0608-500 µg, ATGP0608-1 mg, ATGP0608-10, ATGP0608-20, ATGP0608-50, ATGP0608-100, ATGP0608-250, ATGP0608-500, ATGP0608-1

Tubaro C., et al. (2010) J Cell Physiol. 223 (1) :270-82.; ; Shin EJ., et al. (2009) J Neurosci Res. 87 (16) :3679-86.;

Fujiya A, et al. The role of S100B in the interaction between adipocytes and macrophages. (Obesity (Silver Spring) . 2013) {https://pubmed.ncbi.nlm.nih.gov/23804363/}; ; Hosokawa K, et al. S100B impairs glycolysis via enhanced poly (ADP-ribosyl) ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells. (Am J Physiol Endocrinol Metab. 2017) {https://pubmed.ncbi.nlm.nih.gov/28174179/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Cell Cycle

NP_033141

P50114

Mouse

MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDEDGDGECD FQEFMAFVAM VTTACHEFFE HE