Recombinant muis Sepiapterine reductase/SPR-eiwit

Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Sepiapterin reductase, AA409688, Gm10328

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

30.3 kDa (285aa) confirmed by MALDI-TOF

Spr, Sepiapterin reductase, AA409688, Gm10328, ATGP3652-10 µg, ATGP3652-20 µg, ATGP3652-50 µg, ATGP3652-100 µg, ATGP3652-250 µg, ATGP3652-500 µg, ATGP3652-1 mg, ATGP3652-10, ATGP3652-20, ATGP3652-50, ATGP3652-100, ATGP3652-250, ATGP3652-500, ATGP3652-1

Tobin JE., et al. (2007) . Brain Res. 30; ;1139:42-7.; ; Ohye T., et al. (1998) . Biochem Biophys Res Commun. 251 (2) :597-602.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 10% glycerol

Redox Proteins

NP_035597

Q91XH5

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQ KDTFQSGAHV DFYDC