Recombinant muis SF20/MYDGF-eiwit

Mydgf, also known as Myeloid-derived growth factor, is a bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and repair after myocardial infarction. It stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Mydgf-deficient mice develop larger infarct scars and more severe contractile dysfunction compared to wild-type mice. Recombinant mouse Mydgf, fused to His-tag at N-termineus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myeloid-derived growth factor, D17Wsu104e, Il25, Ly6elg

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

18.1 kDa (165aa) Confirmed by MALDI-TOF

Mydgf, ATGP3689-10 µg, ATGP3689-20 µg, ATGP3689-50 µg, ATGP3689-100 µg, ATGP3689-250 µg, ATGP3689-500 µg, ATGP3689-1 mg, ATGP3689-10, ATGP3689-20, ATGP3689-50, ATGP3689-100, ATGP3689-250, ATGP3689-500, ATGP3689-1

Korf-Klingebiel M., et al. (2015) Nat Med. 21 (2) :140-9; ; Weiler T., et al. (2007) Arthritis Res Ther. 9 (2) :R30.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Hormones & Cytokines

NP_543027

Q9CPT4

Mouse

VSEPTTV PFDVRPGGVV HSFSQDVGPG NKFTCTFTYA SQGGTNEQWQ MSLGTSEDSQ HFTCTIWRPQ GKSYLYFTQF KAELRGAEIE YAMAYSKAAF ERESDVPLKS EEFEVTKTAV SHRPGAFKAE LSKLVIVAKA ARSEL