Recombinant muis sFRP-2/SFRP2-eiwit

SFRP-2, also known as Secreted apoptosis-related protein 1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. sFRP-2 can exert either positive or negative effects on Wnt signaling. At physiological concentrations, it also inhibits BMP-induced effects. It plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation. It is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity. Recombinant mouse sFRP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

SFRP-2, sFRP2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, Sdf5, sdf, SARP-1, SARP1, Protein SDF5, FRP-2secreted apoptosis related protein 1, AI851596

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

32.1kDa (280aa)

, ATGP3973-10 µg, ATGP3973-20 µg, ATGP3973-50 µg, ATGP3973-100 µg, ATGP3973-250 µg, ATGP3973-500 µg, ATGP3973-1 mg, ATGP3973-10, ATGP3973-20, ATGP3973-50, ATGP3973-100, ATGP3973-250, ATGP3973-500, ATGP3973-1

Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645-35653; ; He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110-21115;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Signal Transduction

NP_033170.1

P97299

Mouse

Ala

LFLFGQP DFSYKRSNCK PIPANLQLCH GIEYQNMRLP NLLGHETMKE VLEQAGAWIP LVMKQCHPDT KKFLCSLFAP VCLDDLDETI QPCHSLCVQV KDRCAPVMSA FGFPWPDMLE CDRFPQDNDL CIPLASSDHL LPATEEAPKV CEACKTKNED DNDIMETLCK NDFALKIKVK EITYINRDTK IILETKSKTI YKLNGVSERD LKKSVLWLKD SLQCTCEEMN DINAPYLVMG QKQGGELVIT SVKRWQKGQR EFKRISRSIR KLQC