Recombinant muis SOD2/Mn-SOD eiwit

Sod2 also known as superoxide dismutase [Mn], mitochondrial, is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant mouse Sod2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase [Mn], mitochondrial, superoxide dismutase 2, mitochondrial, MnSOD, Sod-2

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.6 kDa (221aa) confirmed by MALDI-TOF

SOD2, Mn-SOD, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase [Mn], mitochondrial , superoxide dismutase 2, mitochondrial, MnSOD, Sod-2, ATGP3076-10 µg, ATGP3076-20 µg, ATGP3076-50 µg, ATGP3076-100 µg, ATGP3076-250 µg, ATGP3076-500 µg, ATGP3076-1 mg, ATGP3076-10, ATGP3076-20, ATGP3076-50, ATGP3076-100, ATGP3076-250, ATGP3076-500, ATGP3076-1

Cheng Y., et al. (2014) J. Neuroimmunol. 269 (1-2), 38-43

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Redox Proteins

NP_038699

P09671

Mouse

MGSSHHHHHH SSGLVPRGSH MGSKHSLPDL PYDYGALEPH INAQIMQLHH SKHHAAYVNN LNATEEKYHE ALAKGDVTTQ VALQPALKFN GGGHINHTIF WTNLSPKGGG EPKGELLEAI KRDFGSFEKF KEKLTAVSVG VQGSGWGWLG FNKEQGRLQI AACSNQDPLQ GTTGLIPLLG IDVWEHAYYL QYKNVRPDYL KAIWNVINWE NVTERYTACK K