Recombinant muis SPARC-achtig 1/SPARCL1-eiwit

SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Recombinant Mouse SPARCL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

71.7 kDa (642aa)

SPARC-like1, SPARCL1, ATGP3891-10 µg, ATGP3891-20 µg, ATGP3891-50 µg, ATGP3891-100 µg, ATGP3891-250 µg, ATGP3891-500 µg, ATGP3891-1 mg, ATGP3891-10, ATGP3891-20, ATGP3891-50, ATGP3891-100, ATGP3891-250, ATGP3891-500, ATGP3891-1, ATGP3891-10 µg, ATGP3891-20 µg, ATGP3891-50 µg, ATGP3891-100 µg, ATGP3891-250 µg, ATGP3891-500 µg, ATGP3891-1 mg, ATGP3891-10, ATGP3891-20, ATGP3891-50, ATGP3891-100, ATGP3891-250, ATGP3891-500, ATGP3891-1

Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991-996.; ; Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735-748.

Hattori T, et al. Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex. (bioRxiv. 2021) {https://doi.org/10.1101/2021.12.23.474051}; ; Hattori T, et al. Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory. (EMBO J. 2023) {https://pubmed.ncbi.nlm.nih.gov/37357972/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cancer

NP_034227

P70663

Mouse

Ile17