Recombinant muis SPARC eiwit

SPARC, also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin. It is a major bone-related protein that is also present in nonmineralized tissues and in platelets and is matricellular molecule regulating interactions between cells and their surrounding extracellular matrix (ECM) . This protein is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. It shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. It is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis. Recombinant mouse SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, Sparc

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

<1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

33.3kDa (291aa)

, ATGP3945-10 µg, ATGP3945-20 µg, ATGP3945-50 µg, ATGP3945-100 µg, ATGP3945-250 µg, ATGP3945-500 µg, ATGP3945-1 mg, ATGP3945-10, ATGP3945-20, ATGP3945-50, ATGP3945-100, ATGP3945-250, ATGP3945-500, ATGP3945-1

Mizuho Fukunaga-Kalabi., et al, (2008) Cancer Microenviron. 1:93-102.; ; Katarina Kos., et al, (2010) Nat Rev Endocrinol. 6:225-235.; ; Sharon L I Wong., et al, (2017) Br J Pharmacol. 174:3-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_033268

P07214

Mouse

Ala