Recombinant muis STI1/STIP1-eiwit

Stip1, also known as stress induced phosphoprotein 1, belongs to the large group of co-chaperones. The main function of Stip1 is to link Hsp70 and Hsp90 together. Stip1 also modulates the chaperone activities of the linked proteins and possibly interacts with other chaperones and proteins. It forms a complex with HSC70 and HSPCA/HSP-86 and HSPCB/HSP-84, as well as interacting with PACRG. Recombinant mouse Stip1 was expressed in E. coli and purified by using conventional chromatography techniques.

Stress-induced-phosphoprotein 1, Hsc70/Hsp90-organizing protein, Hop, mSTI1, IEF SSP 3521, p60

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

64.7 kDa (563aa) confirmed by MALDI-TOF

Stress-induced-phosphoprotein 1, Stress induced phosphoprotein 1, Stip1, Stip 1, Sti1, Sti 1, p60, mSTI1, IEF SSP 3521, Hsp90 organizing protein, Hsc70/Hsp90-organizing protein, Hsc70/Hsp90 organizing protein, Hsc70 organizing protein, Hop, ATGP3526-10 µg, ATGP3526-20 µg, ATGP3526-50 µg, ATGP3526-100 µg, ATGP3526-250 µg, ATGP3526-500 µg, ATGP3526-1 mg, ATGP3526-010, ATGP3526-020, ATGP3526-050, ATGP3526-100, ATGP3526-250, ATGP3526-500, ATGP3526-01M

Johnson BD., et al. (1998) J Biol Chem. 273 (6) :3679-86.; ; Van Der Spuy J., et al. (2001) Protein Expr Purif. 21 (3) :462-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Heat Shock Proteins

NP_058017

Q60864

Mouse

MEQVNELKEK GNKALSAGNI DDALQCYSEA IKLDPQNHVL YSNRSAAYAK KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNLQLKE GLQNMEARLA ERKFMNPFNL PNLYQKLEND PRTRSLLSDP TYRELIEQLQ NKPSDLGTKL QDPRVMTTLS VLLGVDLGSM DEEEEAATPP PPPPPKKEPK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDKALKHYDR AKELDPTNMT YITNQAAVHF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ QAEKILKEQE RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PRDAKLYSNR AACYTKLLEF QLALKDCEEC IQLEPTFIKG YTRKAAALEA MKDYTKAMDV YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR