Recombinant muis TIM-3/HAVCR2-eiwit

TIM-3, also known as HAVCR2 (epatitis A virus cellular receptor 2), belongs to TIM family cell surface receptor proteins.TIM-3 is up‑regulated on several populations of activated myeloid cells and T cells. It is an immune checkpoint and together with other inhibitory receptors including PD-1and LAG3 mediate the CD8+ T-cell exhaustion. Also, it is primarily activated by galectin-9. The engagement leads to stimulation of an influx of calcium to intracellular space and induction of programmed cell death, apoptosis. As a consequence, a suppression of Th1 and Th17 responses and induction of immune tolerance occurs. Recombinant mouse TIM-3, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, TIM-3, T-cell membrane protein 3, CD366

HEK293

Human, Mouse

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

46.3kDa (413aa)

CD366, Hepatitis A virus cellular receptor 2 homolog, T cell immunoglobulin and mucin domain-containing protein 3, T cell immunoglobulin mucin receptor 3, T cell membrane protein 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM 3, TIM3, TIM-3, TIMD 3, TIMD3, TIMD-3, ATGP3992-10 µg, ATGP3992-20 µg, ATGP3992-50 µg, ATGP3992-100 µg, ATGP3992-250 µg, ATGP3992-500 µg, ATGP3992-1 mg, ATGP3992-010, ATGP3992-020, ATGP3992-050, ATGP3992-100, ATGP3992-250, ATGP3992-500, ATGP3992-01M

Ana C Anderson., et al, (2016) Immunity. 44:989-1004.; ; Ruihan Tang., et al, (2019) Semin Immunol. 42:101302.; ; Huang YH., (2015) Nature. 517:386–390.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_599011.2

Q8VIM0

Human, Mouse

Arg20