Recombinant muis Trombopoëtine-eiwit

Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Megakaryocytopoiesis is the cellular development process that leads to platelet production. It is a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Recombinant mouse Thrombopoietin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Mouse Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤4 ng/mL.

Thpo, C-mpl ligand, ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1

HEK293

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4 ng/mL.

36.4 kDa (341aa)

Thrombopoietin, ATGP3930-10 µg, ATGP3930-20 µg, ATGP3930-50 µg, ATGP3930-100 µg, ATGP3930-250 µg, ATGP3930-500 µg, ATGP3930-1 mg, ATGP3930-10, ATGP3930-20, ATGP3930-50, ATGP3930-100, ATGP3930-250, ATGP3930-500, ATGP3930-1

Jenkins JM., et al. (2007) Blood. 109:4739-4741.; ; Hitchcock IS., et al. (2014) Br J Haemato. 165:259-268.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_033405

P40226

Mouse

Ser22