Recombinant muis Tyrosine Hydroxylase eiwit

Th, also known as tyrosine 3-monooxygenase, is a rate-limiting enzyme in catecholamine synthesis. It uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. It regulates dopamine (DA) neurotransmission at the biosynthesis and reuptake steps. It plays an important role in the physiology of adrenergic neurons. It effects overexpression in lymphocytes on the differentiation and function of T helper cells. Recombinant mouse Th, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tyrosine 3-monooxygenase, Th, tyrosine hydroxylase

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

57 kDa (507aa)

Th, Tyrosine 3-monooxygenase, Th, tyrosine hydroxylase, ATGP3438-10 µg, ATGP3438-20 µg, ATGP3438-50 µg, ATGP3438-100 µg, ATGP3438-250 µg, ATGP3438-500 µg, ATGP3438-1 mg, ATGP3438-10, ATGP3438-20, ATGP3438-50, ATGP3438-100, ATGP3438-250, ATGP3438-500, ATGP3438-1

Yamada K., et al. (1992) Histochemistry. 97:201-206.; ; Daubner SC., et al. (2011) Arch Biochem Biophys. 508:1-12.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_033403.1

P24529

Mouse

Ala

MPTPSAS SPQPKGFRRA VSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLF SLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVR SAREDKVPWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTK EEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYRE DSIPQLEDVS HFLKERTGFQ LRPVAGLLSA RDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL GASDEEIEKL STVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSES FSDAKDKLRN YASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI S