Recombinant muis VAMP-2-eiwit

Vamp2, also known as Vesicle-associated membrane protein 2, is involved in the targeting and/or fusion of transport vesicles to their target membrane. It modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Vamp2 proteins localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. This proteins also known to mediate cAMP-stimulated exocytosis in nerve cells and in renal cells of the juxtaglomerular apparatus. Recombinant mouse Vamp2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Vesicle-associated membrane protein 2, Syb-2, sybII

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.8 kDa (118aa) Confirmed by MALDI-TOF

Vamp 2, Vamp2, Vesicle-associated membrane protein 2, Syb-2, Syb 2, Syb2, sybII, ATGP3555-10 µg, ATGP3555-20 µg, ATGP3555-50 µg, ATGP3555-100 µg, ATGP3555-250 µg, ATGP3555-500 µg, ATGP3555-1 mg, ATGP3555-010, ATGP3555-020, ATGP3555-050, ATGP3555-100, ATGP3555-250, ATGP3555-500, ATGP3555-01M

Scales SJ., et al. (2002) J Biol Chem. 277 (31) :28271-9.; ; Mendez M., et al. (2011) J Biol Chem. 286 (32) :28608-18

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM EDTA, 0.1mM PMSF, 10% glycerol

Neuroscience

NP_033523.1

P63044

Mouse

MSATAA TVPPAAPAGE GGPPAPPPNL TSNRRLQQTQ AQVDEVVDIM RVNVDKVLER DQKLSELDDR ADALQAGASQ FETSAAKLKR KYWWKNLK