Recombinant muis VEGF 120/VEGFA-eiwit

VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. Recombinant VEGF-A protein was expressed in E. coli and purified by using conventional chromatography techniques.

Mouse VEGF in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.

Vascular endothelial growth factor A isoform 3, Vegf-a, Vegf120, Vpf

E.coli

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤ 15 ng/mL.

16.3 kDa (141aa) confirmed by MALDI-TOF

Vascular endothelial growth factor A isoform 3, Vegf-a, Vegf120, Vpf, ATGP0389-10 µg, ATGP0389-20 µg, ATGP0389-50 µg, ATGP0389-100 µg, ATGP0389-250 µg, ATGP0389-500 µg, ATGP0389-1 mg, ATGP0389-10, ATGP0389-20, ATGP0389-50, ATGP0389-100, ATGP0389-250, ATGP0389-500, ATGP0389-1

Gerber H., et al. (2002) Nature. 417: 954-958.; ; Shibuya M., et al. (2006) J Biochem Mol Biol. 39:469-478.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Growth factors

NP_001020428.1

Q00731-3

Mouse

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