Recombinant muis VPS29 eiwit

Vacuolar protein sorting 29, also known as VPS29, belongs to a group of genes coding for vacuolar protein sorting (VPS) proteins that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. It is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. Also, VPS29 may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Recombinant Mouse VPS29 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

VPS29 retromer complex component, Vacuolar protein sorting-associated protein 29, Vesicle protein sorting 29, PEP11, DC7, DC15

E.coli

Mouse

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.2 kDa (207aa) confirmed by MALDI-TOF

VPS29, Vacuolar protein sorting 29, PEP11, ATGP1468-10 µg, ATGP1468-20 µg, ATGP1468-50 µg, ATGP1468-100 µg, ATGP1468-250 µg, ATGP1468-500 µg, ATGP1468-1 mg, ATGP1468-10, ATGP1468-20, ATGP1468-50, ATGP1468-100, ATGP1468-250, ATGP1468-500, ATGP1468-1

Edgar AJ., et al. (2000) Biochem Biophys Res Commun. 277 (3) :622-30.; ; Wanq D., et al. (2005) J Biol Chem. 280 (24) :22962-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Transportation

NP_062754

Q9QZ88

Mouse

MGSSHHHHHH SSGLVPRGSH MGSHMMLVLV LGDLHIPHRC NSLPAKFKKL LVPGKIQHIL CTGNLCTKES YDYLKTLAGD VHIVRGDFDE NLNYPEQKVV TVGQFKIGLI HGHQVIPWGD MASLALLQRQ FDVDILISGH THKFEAFEHE NKFYINPGSA TGAYNALETN IIPSFVLMDI QASTVVTYVY QLIGDDVKVE RIEYKKS