Recombinant muisAminopeptidase N/CD13 eiwit

Anpep, also known as aminopeptidase N, is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. It plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. This protein is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. It has a role in angiogenesis and promote cholesterol crystallization and in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity. Recombinant mouse Anpep, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of H-Ala-AMC to Alanine and AMC per minute at pH7.5 at 25C.

Anpep, AP-M, AP-N, Apn, Cd13, P150, mAPN, Alanyl aminopeptidase, Aminopeptidase M, Membrane protein p161, Microsomal aminopeptidase, CD13, Lap-1, Lap1, aminopeptidase N

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of H-Ala-AMC to Alanine and AMC per minute at pH7.5 at 25C.

107.5 kDa (943aa)

Aminopeptidase N, CD13, ATGP3925-10 µg, ATGP3925-20 µg, ATGP3925-50 µg, ATGP3925-100 µg, ATGP3925-250 µg, ATGP3925-500 µg, ATGP3925-1 mg, ATGP3925-10, ATGP3925-20, ATGP3925-50, ATGP3925-100, ATGP3925-250, ATGP3925-500, ATGP3925-1

Azimi A., et al. (2017) Cell Death Dis. 8:e3029.; ; S첩rensen KD., et al. (2013) Br J Cancer. 108:420-428.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_032512

P97449

Mouse

Ala

YAQEKNR NAENSATAPT LPGSTSATTA TTTPAVDESK PWNQYRLPKT LIPDSYRVIL RPYLTPNNQG LYIFQGNSTV RFTCNQTTDV IIIHSKKLNY TLKGNHRVVL RTLDGTPAPN IDKTELVERT EYLVVHLQGS LVEGRQYEMD SQFQGELADD LAGFYRSEYM EGDVKKVVAT TQMQAADARK SFPCFDEPAM KAMFNITLIY PNNLIALSNM LPKESKPYPE DPSCTMTEFH STPKMSTYLL AYIVSEFKNI SSVSANGVQI GIWARPSAID EGQGDYALNV TGPILNFFAQ HYNTSYPLPK SDQIALPDFN AGAMENWGLV TYRESSLVFD SQSSSISNKE RVVTVIAHEL AHQWFGNLVT VAWWNDLWLN EGFASYVEYL GADYAEPTWN LKDLMVLNDV YRVMAVDALA SSHPLSSPAD EIKTPDQIME LFDSITYSKG ASVIRMLSSF LTEDLFKKGL SSYLHTYQYS NTVYLDLWEH LQKAVNQQTA VQPPATVRTI MDRWILQMGF PVITVNTNTG EISQKHFLLD SKSNVTRPSE FNYIWIAPIP FLKSGQEDHY WLDVEKNQSA KFQTSSNEWI LLNINVTGYY LVNYDENNWK KLQNQLQTDL SVIPVINRAQ IIHDSFNLAS AKMIPITLAL DNTLFLVKEA EYMPWQAALS SLNYFTLMFD RSEVYGPMKR YLKKQVTPLF FYFQNRTNNW VNRPPTLMEQ YNEINAISTA CSSGLKECRD LVVELYSQWM KNPNNNTIHP NLRSTVYCNA IAFGGEEEWN FAWEQFRNAT LVNEADKLRS ALACSKDVWI LNRYLSYTLN PDYIRKQDTT STIISIASNV AGHPLVWDFV RSNWKKLFEN YGGGSFSFAN LIQGVTRRFS SEFELQQLEQ FKADNSATGF GTGTRALEQA LEKTRANIDW VKENKDAVFK WFTENSS