Recombinant muistestican 3/SPOCK3-eiwit

Spock3, also known as testican-3, is a nervous system-expressed heparan sulfate proteoglycan belonging to a subgroup of the BM-40/SPARC/osteonectin family, the role of which in brain development is unclear. It inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. It is mostly confined to the developmental stage of the brain. Recombinant mouse Spock3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Testican-3, Spock3, 2900045C01Rik, AI428471, mKIAA4039

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

47.9 kDa (423aa)

Testican3, SPOCK3, Testican-3, Spock3, 2900045C01Rik, AI428471, mKIAA4039, ATGP3359-10 µg, ATGP3359-20 µg, ATGP3359-50 µg, ATGP3359-100 µg, ATGP3359-250 µg, ATGP3359-500 µg, ATGP3359-1 mg, ATGP3359-10, ATGP3359-20, ATGP3359-50, ATGP3359-100, ATGP3359-250, ATGP3359-500, ATGP3359-1

Hartmann U., et al. (2013) J Neurochem. 125:399-409.; ; Yamamoto A., et al. (2014) Dev Neurosci. 36:381-395.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001239549

Q8BKV0

Mouse

Ala22

AAAAVAVAGG RSDGGNFLDE KQWLTTISQY DKEVGQWNKF RDEVEDDYFR TWNPGKPFDQ ALDPAKDPCL KTKCSRHKVC ITQDAQTALC ISHRRLTHSM KEVGGSHKQW RGLPSSTCKP CPIAYASPVC GSDGHSYSSQ CKLEYQACVL GKQISIKCEG RCPCPSDKSM NIGRNVKRAC SDLEFREVAN RLRDWFKALH ESGSQNKKTK ALLRPERSRF DTSILPICKD SLGWMFNRLD TNYDLLLDQS ELGSIYLDKN EQCTKAFFNS CDTYKDSLIS NNEWCYCFQR QQDPPCHTEL SNIQKRQGIK KLLGQYIPLC DEDGYYKPTQ CHGSVGQCWC VDRYGNEVVG SRINGVADCA IDFEISGDFA SGDFREWTDD EGEEDDIMND KDDIEDDDED EGDDDDDGDV HDGYILEHHH HHH