Recombinant muizen NMNAT-1 eiwit

Nmnat1, also known as nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) of nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant mouse Nmnat1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Nicotinamide nucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, NMN adenylyltransferase 1, NaMN adenylyltransferase 1, Leber's congenital amaurosis 9, PNAT1

E.coli

Mouse

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

34.7 kDa (308aa) confirmed by MALDI-TOF

Nmnat1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, 2610529L11Rik, 5730441G13Rik, D4Cole1e, nmnat, ATGP3606-10 µg, ATGP3606-20 µg, ATGP3606-50 µg, ATGP3606-100 µg, ATGP3606-250 µg, ATGP3606-500 µg, ATGP3606-1 mg, ATGP3606-10, ATGP3606-20, ATGP3606-50, ATGP3606-100, ATGP3606-250, ATGP3606-500, ATGP3606-1

Emanuelli M., et al. (2001) J Biol Chem. 276 (1) :406-12; ; Zhou T., et al. (2002) J Biol Chem. 277 (15) 13148-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM EDTA

Neuroscience

NP_597679

Q9EPA7

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMDSSKKT EVVLLACGSF NPITNMHLRL FELAKDYMHA TGKYSVIKGI ISPVGDAYKK KGLIPAHHRI IMAELATKNS HWVEVDTWES LQKEWVETVK VLRYHQEKLA TGSCSYPQSS PALEKPGRKR KWADQKQDSS PQKPQEPKPT GVPKVKLLCG ITNDISSTKI RRALRRGQSI RYLVPDLVQE YIEKHELYNT ESEGRNAGVT LAPLQRNAAE AKHNHSTL