Recombinant muizen UCHL3-eiwit

Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus, solitaris, and area postrema, and in working memory. Recombinant mouse Uchl3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin thioesterase L3, UCH-L3

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 9,000pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)

HUMAN UCHL3, Ubiquitin carboxyl terminal esterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCHL3, UCH-L3, UCHL3 HUMAN, UCHL3_HUMAN, UCHL3HUMAN, ATGP3413-10 µg, ATGP3413-20 µg, ATGP3413-50 µg, ATGP3413-100 µg, ATGP3413-250 µg, ATGP3413-500 µg, ATGP3413-1 mg, ATGP3413-010, ATGP3413-020, ATGP3413-050, ATGP3413-100, ATGP3413-250, ATGP3413-500, ATGP3413-01M

Mtango NR., et al. (2012) J. Cell. Physiol. 227:2022-2029.; ; Dennissen FJ., et al. (2011) FEBS Lett. 585:2568-2574.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Ubiquitination

NP_057932

Q9JKB1

Mouse

Met1

MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH