Recombinant Porcine GM-CSF-eiwit

GM-CSF, also known as granulocyte macrophage colony-stimulating factor, is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant porcine GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Porcine GM-CSF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/mL.

Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GM-CSF, GMCSF

E.coli

Pocrine

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/mL.

16.6 kDa (148aa), confirmed by MALDI-TOF

Granulocyte Macrophage Colony Stimulating Factor, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, MGI1GM, Molgramostin, Pluripoietin-a, Sargramostim, ATGP3935-10 µg, ATGP3935-20 µg, ATGP3935-50 µg, ATGP3935-100 µg, ATGP3935-250 µg, ATGP3935-500 µg, ATGP3935-1 mg, ATGP3935-10, ATGP3935-20, ATGP3935-50, ATGP3935-100, ATGP3935-250, ATGP3935-500, ATGP3935-1

Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80. Wong GG, et al (1985) Science, 228, 810-815.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH7.4)

Growth factors

NP_999283

Q29118

Pocrine

APTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK