Recombinant RAG2- monoklonaal antilichaam

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V (D) J recombination. V (D) J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V (D) J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps.

RAG2

P55895

Human; Mouse

Recombinant human RAG2 fragment

RAG; RAG2; RAG-2; recombination activating 2; recombination activating gene 2

A350

Unconjugated

WB

Epigenetics and Nuclear Signaling

Protein A purified

1 mg/mL

WB 1:1000-1:10000

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Ice bag

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

https://789.bio/eb/PxFVDYkM

62 kDa

62 kDa

IgG, κ