Recombinant-rat ALT1/GPT1-eiwit

Gpt also known as Alanine aminotransferase 1, is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate. The Gpt is widely distributed in various tissues from animals and even in some kind of plants. It is suggested that c-ALT is associated to the utilization of pyruvate in glycolysis and m-ALT is involved in the conversion of alanine to pyruvate for gluconeogenesis. Recombinant rat Gpt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Specific activity is > 60units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

Alanine aminotransferase 1

E.coli

Rat

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 60unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

57.5 kDa (519aa)

ALT1, GPT1, Alanine aminotransferase 1, ATGP3161-10 µg, ATGP3161-20 µg, ATGP3161-50 µg, ATGP3161-100 µg, ATGP3161-250 µg, ATGP3161-500 µg, ATGP3161-1 mg, ATGP3161-10, ATGP3161-20, ATGP3161-50, ATGP3161-100, ATGP3161-250, ATGP3161-500, ATGP3161-1

Yang RZ., et al. (2009) Hepatology. 49 (2) :598-607.; ; Ishiguro M., et al. (1991) Biochemistry. 30 (43) :10451-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_112301

P25409

Rat

MGSSHHHHHH SSGLVPRGSH MGSMASRVND QSQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS