Recombinant rat CNTFR alfa-eiwit

Cntfr, also known as ciliary neurotrophic factor receptor subunit alpha, is a member of the type 1 cytokine receptor family. It mediates the biological action of CNTF. CNTF is a polypeptide hormone that supports the survival of neurons of the peripheral sensory, sympathetic, and ciliary ganglia at various stages in their development. CNTFR and its cognate ligand support the survival of neurons. The CNTF receptor complex is most closely related to, and shares subunits with the receptor complexes for interleukin-6 and leukemia inhibitory factor. The specificity conferring alpha subunit of the CNTF complex (CNTFR alpha), is extremely well conserved across species, and has a distribution localized predominantly to the nervous system and skeletal muscle. Recombinant rat Cntfr, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ciliary neurotrophic factor receptor subunit alpha, CNTFR, CNTF receptor subunit alpha, CNTFR-alpha

Baculovirus

Rat

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

36.9 kDa (328aa)

Ciliary neurotrophic factor receptor subunit alpha, CNTFR, CNTF receptor subunit alpha, CNTFR-alpha, CNTF R a, CNTFR alpha, ATGP3474-10 µg, ATGP3474-20 µg, ATGP3474-50 µg, ATGP3474-100 µg, ATGP3474-250 µg, ATGP3474-500 µg, ATGP3474-1 mg, ATGP3474-010, ATGP3474-020, ATGP3474-050, ATGP3474-100, ATGP3474-250, ATGP3474-500, ATGP3474-01M

Dutt K., et al. (2010) In Vitro Cell Dev Biol Anim. 46:635-646.; ; Clatterbuck RE., et al. (1993) Proc Natl Acad Sci U S A. 15:2222-2226.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_001003929

Q08406

Rat

Gln23

QKHSPQEAPH VQYERLGTDV TLPCGTASWD AAVTWRVNGT DLAPDLLNGS QLILRSLELG HSGLYACFHR DSWHLRHQVL LHVGLPPREP VLSCRSNTYP KGFYCSWHLS APTYIPNTFN VTVLHGSKMM VCEKDPALKN RCHIRYMHLF STIKYKVSIS VSNALGHNTT AITFDEFTIV KPDPPENVVA RPVPSNPRRL EVTWQTPSTW PDPESFPLKF FLRYRPLILD QWQHVELSNG TAHTITDAYA GKEYIIQVAA KDNEIGTWSD WSVAAHATPW TEEPRHLTTE AQAPETTTST TSSLAPPPTT KICDPGELSS LEHHHHHH