Recombinant rat Cyclophiline F/PPIF-eiwit

Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP) . It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CYPD, Cyclophilin F, Rotamase F

E.coli

Rat

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

21.2Da (200aa) confirmed by MALDI-TOF

Cyclophilin D, Cyclophilin F, CYP D, CYPD, CYP-D, mitochondrial, Peptidyl-prolyl cis-trans isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase, Ppif, ATGP3496-10 µg, ATGP3496-20 µg, ATGP3496-50 µg, ATGP3496-100 µg, ATGP3496-250 µg, ATGP3496-500 µg, ATGP3496-1 mg, ATGP3496-010, ATGP3496-020, ATGP3496-050, ATGP3496-100, ATGP3496-250, ATGP3496-500, ATGP3496-01M

Connern C.P., et al. (1992) Biochem. J. 284:381-385.; ; Lin DT., et al. (2002) J Biol Chem. 23:277 (34) :31134-41.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_758443

P29117

Rat

MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS