Recombinant rat Erytropoëtine/EPO-eiwit

EPO, also known as erythropoietin, is a member of t the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant rat Erythropoietin/EPO Protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Rat EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.

Erythropoietin, erythropoietin isoform 1 precursor, Epo

HEK293

Rat

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.

19.6kDa (175aa)

, ATGP4000-10 µg, ATGP4000-20 µg, ATGP4000-50 µg, ATGP4000-100 µg, ATGP4000-250 µg, ATGP4000-500 µg, ATGP4000-1 mg

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Angiogenesis, Cardiovascular

NP_058697.1

P29676

Rat

Asp

APPRLIC DSRVLERYIL EAKEAENVTM GCAEGPRLSE NITVPDTKVN FYAWKRMKVE EQAVEVWQGL SLLSEAILQA QALQANSSQP PESLQLHIDK AISGLRSLTS LLRVLGAQKE LMSPPDATQA APLRTLTADT FCKLFRVYSN FLRGKLKLYT GEACRRGDR