Recombinant rat IL-1 alfa/IL1A-eiwit

IL-1 alpha also known as interleukin-1 alpha. IL-1 alpha belongs to the interleukin 1 cytokine family. IL-1 alpha is part of Interleukin-1. IL1 stimulates thymocyte proliferation by inducing IL2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1 proteins are involved in the inflammatory response, especially IL1A is an early and important mediator of inflammation after injury. Recombinant rat IL1A, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Rat IL-1 alpha in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.

Interleukin-1 alpha precursor, Interleukin-1 alpha, IL1F1, IL-1A, IL-1 alpha

E.coli

Rat

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 20 pg/ml.

20.2 kDa (179aa) confirmed by MALDI-TOF

IL 1 A, IL 1A, IL-1 alpha, IL1A, IL-1A, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, Preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, Hematopoietin 1, Pro interleukin 1 alpha, ATGP3846-10 µg, ATGP3846-20 µg, ATGP3846-50 µg, ATGP3846-100 µg, ATGP3846-250 µg, ATGP3846-500 µg, ATGP3846-1 mg, ATGP3846-010, ATGP3846-020, ATGP3846-050, ATGP3846-100, ATGP3846-250, ATGP3846-500, ATGP3846-01M

Brough D., et al. (2015) IUBMB Life. doi: 10.1002; ; Hermann E., et al. (1985) Z Rheumatol. 44 (5) : 207-12.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_058715

P16598

Rat

SAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS