Recombinant rat IL-6 eiwit

Il6, also known as interleukin 6, is an alpha-helical phosphorylated and glycosylated cytokine. This protein is important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism and cancer progression. Also, it can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells. Recombinant rat Il6, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.

Interleukin 6, Il6, Ifnb2, ILg6, Interferon beta 2

Baculovirus

Rat

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.

22.8 kDa (196aa)

IL6, ATGP3711-10 µg, ATGP3711-20 µg, ATGP3711-50 µg, ATGP3711-100 µg, ATGP3711-250 µg, ATGP3711-500 µg, ATGP3711-1 mg, ATGP3711-10, ATGP3711-20, ATGP3711-50, ATGP3711-100, ATGP3711-250, ATGP3711-500, ATGP3711-1

Garbers C., et al, (2012) Cytokine Growth Factor Rev. 23:85-97.; ; Northemann W., et al, (1989) J. Biol. Chem. 264:16072-16082.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Hormones & Cytokines

NP_036721

P20607

Rat

Ala

FPTSQVR RGDFTEDTTH NRPVYTTSQV GGLITYVLRE ILEMRKELCN GNSDCMNSDDALSENNLKLP EIQRNDGCFQ TGYNQEICLL KICSGLLEFR FYLEFVKNNL QDNKKDKARVIQSNTETLVH IFKQEIKDSY KIVLPTPTSN ALLMEKLESQ KEWLRTKTIQ LILKALEEFL KVTMRSTRQT