Recombinant rat NM23-H2/NME2-eiwit

Nme2 also known as nucleoside diphosphate kinaseB is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. Nme2 is a ubiquitous enzyme that catalyses phosphorylation of nucleoside 5-diphosphate (NDP) to the corresponding triphosphate (NTP), following a Ping-Pong mechanism which includes the formation of a phosphohistidine intermediate. Recombinant rat Nme2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleoside diphosphate kinase B, NDKB, nm23-2, p18-12d, NME/NM23 nucleoside diphosphate kinase 2, NDP kinase B, Histidine protein kinase NDKB, NM23B

E.coli

Rat

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

19.7 kDa (175aa) confirmed by MALDI-TOF

Nme2, Nucleoside diphosphate kinase B, NDKB, nm23-2, p18-12d, ATGP3150-10 µg, ATGP3150-20 µg, ATGP3150-50 µg, ATGP3150-100 µg, ATGP3150-250 µg, ATGP3150-500 µg, ATGP3150-1 mg, ATGP3150-10, ATGP3150-20, ATGP3150-50, ATGP3150-100, ATGP3150-250, ATGP3150-500, ATGP3150-1

Ishikawa N., et al. (1992) J. Biol. Chem. 267 (20) :14366-72.; ; Hemmerich S., et al. (1992) Biochemistry. 31 (19) :4574-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 30% glycerol, 0.15M NaCl

Metabolism

NP_114021

P19804

Rat

MGSSHHHHHH SSGLVPRGSH MGSMANLERT FIAIKPDGVQ RGLVGEIIKR FEQKGFRLVA MKFLRASEEH LKQHYIDLKD RPFFPGLVKY MNSGPVVAMV WEGLNVVKTG RVMLGETNPA DSKPGTIRGD FCIQVGRNII HGSDSVESAE KEIGLWFKPE ELIDYKSCAH DWVYE