Recombinant rat SIRP-alfa/CD172a-eiwit

SIRPA, also known as tyrosine-protein phosphatase non-receptor type substrate 1, is an immunoglobulin-like cell surface receptor for CD47. It supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. It is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Recombinant rat SIRPA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Bit, Ptpns1, SHPS-1, Sirpa

Baculovirus

Rat

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

38.5 kDa (350aa)

SIRP a, CD172a, Bit, Ptpns1, SHPS-1, Sirpa, ATGP3253-10 µg, ATGP3253-20 µg, ATGP3253-50 µg, ATGP3253-100 µg, ATGP3253-250 µg, ATGP3253-500 µg, ATGP3253-1 mg, ATGP3253-10, ATGP3253-20, ATGP3253-50, ATGP3253-100, ATGP3253-250, ATGP3253-500, ATGP3253-1

Fujioka Y., et al. (1996) Mol Cell Biol. 16:6887-6899.; ; Sano S., et al. (1997) FEBS Lett. 411:327-334.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_037148

P97710

Rat