Recombinant Rock2 + Rock1 monoklonaal antilichaam

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity. ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain. ROCK2 is directly cleaved by granzyme B (grB) . Cleavage activates ROCK2 and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction.

ROCK2+ROCK1

O75116; Q13464

Human; Rat; Mouse

Recombinant human ROCK2+ROCK1 fragment

Rho-associated protein kinase 2; ROCK-II; p160ROCK; ROCK-I

A626

Unconjugated

WB; IHC; IF

Protein A purified

1 mg/mL

WB 1:1000; IHC 1:50-1:100; IF 1:50

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Ice bag

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

https://789.bio/eb/SISC3Zu0

161, 158 kDa

161 kDa

IgG, κ