Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-eiwit

An epidemic of acute respiratory syndrome in humans, which appeared in Wuhan, China in December 2019, was caused by a novel coronavirus (SARS-CoV-2) . This disease was named as "Coronavirus Disease 2019" (COVID-19) . This virus shares highly homological sequence with SARS-CoV, and causes acute, highly lethal pneumonia coronavirus disease 2019 (COVID-19) with clinical symptoms similar to those reported for SARS-CoV and MERS-CoV. The genome of this and other emerging pathogenic human CoVs encodes four major structural proteins [spike (S), envelope (E), membrane (M), and nucleocapsid (N) ], approximately 16 nonstructural proteins (nsp1–16), and five to eight accessory proteins. Among them, the S protein plays an essential role in viral attachment, fusion, entry, and transmission. It comprises an N-terminal S1 subunit responsible for virus–receptor binding and a C-terminal S2 subunit responsible for virus–cell membrane fusion. S1 is further divided into an N-terminal domain (NTD) and a receptor-binding domain (RBD) . SARS-CoV-2 and SARS-CoV bind angiotensin-converting enzyme 2 (ACE2) while MERS-CoV binds dipeptidyl peptidase 4 (DPP4), as receptors on the host cell expressing ACE2 (e.g., pneumocytes, enterocytes) or DPP4 (e.g., liver or lung cells including Huh-7, MRC-5, and Calu-3) . During infection, CoV first binds the host cell through interaction between its S1-RBD and the cell membrane receptor, triggering conformational changes in the S2 subunit that result in virus fusion and entry into the target cell. Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

SARS-CoV-2 Spike RBD is coated at 5 µg/mL (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.

Severe acute respiratory syndrome coronavirus 2, COVID-19, COVID-19 virus, COVID19, HCoV-19, Human coronavirus 2019, SARS-2, SARS-CoV2, SARS2, Wuhan coronavirus, SARS-CoV-2 SP RBD, 2019-nCoV SP RBD, 2019-nCoV, 2019-nCoV; Spike RBD Protein

HEK293

SARS-CoV-2 (2019-nCoV)

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# ATGP3963)

26.3kDa (233aa)

Covid-19, covid19, sars, sars2, corona virus, Sars-CoV-2 S1, covid, corona, ATGP3968-10 µg, ATGP3968-20 µg, ATGP3968-50 µg, ATGP3968-100 µg, ATGP3968-250 µg, ATGP3968-500 µg, ATGP3968-1 mg, ATGP3968-10, ATGP3968-20, ATGP3968-50, ATGP3968-100, ATGP3968-250, ATGP3968-500, ATGP3968-1

Wu, F. et al. (2020) Nature. 579:265-269.; ; Ortega, J.T. et al. (2020) EXCLI J. 19:410-417.; ; Wanbo T., et al, (2020) Cell. Mol. Immunol. 17:613-620.; ; Gorbalenya A., et al (2020) Nat. Microbiol. 5:536-544.; ; Tortorici, M.A. and D. Veesler (2019) . Adv. Virus Res. 105:93-116.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Virus

QHD43416.1

P0DTC2

SARS-CoV-2 (2019-nCoV)

Asp

RVQPTE SIVRFPNITN LCPFGEVFNA TRFASVYAWN RKRISNCVAD YSVLYNSASFSTFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV CGPKKSTNLV KNKCVNF