Recombinant SARS-CoV-2 Spike S1 Proteïne (Zijn label)

Source: HEK 293 cells. The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2) . A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein

43740568

Functional Assay

>90% by SDS-PAGE.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles.

Bio-Activity: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL. Endotoxin : < 1.0 EU/μg of the protein by LAL method.

The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1fused with a 6XHis tag at the C-terminus.