Recombinant SARS-CoV RBD-eiwit spike

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

SARS-CoV Spike RBD is coated at 5 µg/mL (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.

E2 glycoprotein precursor, Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Severe acute repiratory Syndrome-related Coronavirus, SARS, SRAS-CoV, SARS-CoV1

Baculovirus

SARS-CoV

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# ATGP3963)

24.7kDa (219aa)

Covid-19, covid19, sars, sars2, Sars-CoV-2 S1, covid, corona, ATGP3960-10 µg, ATGP3960-20 µg, ATGP3960-50 µg, ATGP3960-100 µg, ATGP3960-250 µg, ATGP3960-500 µg, ATGP3960-1 mg, ATGP3960-10, ATGP3960-20, ATGP3960-50, ATGP3960-100, ATGP3960-250, ATGP3960-500, ATGP3960-1

Kukla M., et al, (2020) J Clin Med. 9:1420.; ; Ayouba A., et al, (2020) J Clin Virol. 129:104521.; ; Tortorici, M.A. and D. Veesler (2019) . Adv. Virus Res. 105:93-116.; ; Li F, et al, (2005) Science. 309:1864-1868.; ; Struck AW, et al, (2012) Antiviral Res. 94:288-296.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Virus

NP_828851.1

P59594

SARS-CoV

Ala

RVVPSGD VVRFPNITNL CPFGEVFNAT KFPSVYAWER KKISNCVADY SVLYNSTFFS TFKCYGVSAT KLNDLCFSNV YADSFVVKGD DVRQIAPGQT GVIADYNYKL PDDFMGCVLA WNTRNIDATS TGNYNYKYRY LRHGKLRPFE RDISNVPFSP DGKPCTPPAL NCYWPLNDYG FYTTTGIGYQ PYRVVVLSFE LLNAPATVCG PKL