Recombinant SIGLEC3 Antilichaam / Siale zuurbinding Ig-achtige lectine 3 / CD33

Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P20138

Human

A portion of amino acids 100-300 from human CD33 protein was used as the immunogen for the recombinant SIGLEC3 antibody.

RSIGLEC3/13503

IHC-P

Protein G affinity

Purified

This recombinant SIGLEC3 antibody is available for research use only.

Aliquot the recombinant SIGLEC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant SIGLEC3 antibody should be determined by the researcher.

Cell membrane, Cytoplasm