Recombinant streptomyces Streptavidin eiwit

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Streptavidin, STAV

E.coli

Streptomyces, Streptomyces avidinii

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

15.6 kDa (148aa) confirmed by MALDI-TOF

Streptavidin, Streptavidin, STAV, ATGP2140-10 µg, ATGP2140-20 µg, ATGP2140-50 µg, ATGP2140-100 µg, ATGP2140-250 µg, ATGP2140-500 µg, ATGP2140-1 mg, ATGP2140-10, ATGP2140-20, ATGP2140-50, ATGP2140-100, ATGP2140-250, ATGP2140-500, ATGP2140-1

Alon R., et al. (1990) Biochem. Biophys. Res. Commun. 170:1236-1241; ; Freitag S., et al. (1997) Protein Sci. 6:1157-1166

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Other

CAA27265

P22629

Streptomyces, Streptomyces avidinii

AEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP