Recombinant TLE1 Antilichaam / Transducine-achtige versterker eiwit 1

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Immunohistochemistry (FFPE) : 1-2 µg/mL

Q04724

Human

A recombinant full-length human TLE1 protein was used as the immunogen for the recombinant TLE1 antibody.

RTLE1/13032

IHC-P

Protein G affinity

Purified

This recombinant TLE1 antibody is available for research use only.

Aliquot the recombinant TLE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant TLE1 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human endometrium tissue with recombinant TLE1 antibody (clone rTLE1/13032) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.