Recombinant TNF-alfa-eiwit bij muizen

TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E. coli and purified by using conventional chromatography.

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

E.coli

Mouse

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.06 ng/mL.

17.4 kDa (157aa) confirmed by MALDI-TOF

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin, ATGP3738, ATGP3651-10 µg, ATGP3651-20 µg, ATGP3651-50 µg, ATGP3651-100 µg, ATGP3651-250 µg, ATGP3651-500 µg, ATGP3651-1 mg, ATGP3651-10, ATGP3651-20, ATGP3651-50, ATGP3651-100, ATGP3651-250, ATGP3651-500, ATGP3651-1, ATGP3651-10 µg, ATGP3651-20 µg, ATGP3651-50 µg, ATGP3651-100 µg, ATGP3651-250 µg, ATGP3651-500 µg, ATGP3651-1 mg, ATGP3651-10, ATGP3651-20, ATGP3651-50, ATGP3651-100, ATGP3651-250, ATGP3651-500, ATGP3651-1

Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705; ; Pennica D., et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:6060-6064

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Hormones & Cytokines

NP_038721

P06804

Mouse

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL