Recombinant Tumor Proteïne 53 Antilichaam / p53

P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P04637

Human

A recombinant human full-length TP53 protein was used as the immunogen for the recombinant Tumor Protein 53 antibody.

TP53/8183R

IHC-P

Protein A affinity

Purified

This recombinant Tumor Protein 53 antibody is available for research use only.

Aliquot the recombinant Tumor Protein 53 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the recombinant Tumor Protein 53 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human ovarian carcinoma tissue with recombinant Tumor Protein 53 antibody (clone TP53/8183R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.