Recombinante humane Meprin Een subeenheid alfa/MEP1A-eiwit

MEP1A, also known as meprin A subunit alpha, is a single-pass type I membrane protein which belongs to the peptidase M12A family. It abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. It is capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Recombinant human MEP1A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Meprin A subunit alpha, MEP1A, PPHA, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH alpha

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

67.4 kDa (589aa)

Endopeptidase 2, Endopeptidase2, Endopeptidase-2, MEP1A, Meprin A subunit alpha, N benzoyl L tyrosyl P amino benzoic acid hydrolase subunit alpha, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH A, PPH alpha, PPHA, PPHalpha, ATGP3425-10 µg, ATGP3425-20 µg, ATGP3425-50 µg, ATGP3425-100 µg, ATGP3425-250 µg, ATGP3425-500 µg, ATGP3425-1 mg, ATGP3425-010, ATGP3425-020, ATGP3425-050, ATGP3425-100, ATGP3425-250, ATGP3425-500, ATGP3425-01M

Geurts N., et al. (2012) FEBS Lett. 586:4264-4269.; ; Minder P., et al. (2012) J Biol Chem. 287:35201-35211.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_005579

Q16819

Human

Ala

ADPVPIKYLP EENVHDADFG EQKDISEINL AAGLDLFQGD ILLQKSRNGL RDPNTRWTFP IPYILADNLG LNAKGAILYA FEMFRLKSCV DFKPYEGESS YIIFQQFDGC WSEVGDQHVG QNISIGQGCA YKAIIEHEIL HALGFYHEQS RTDRDDYVNI WWDQILSGYQ HNFDTYDDSL ITDLNTPYDY ESLMHYQPFS FNKNASVPTI TAKIPEFNSI IGQRLDFSAI DLERLNRMYN CTTTHTLLDH CTFEKANICG MIQGTRDDTD WAHQDSAQAG EVDHTLLGQC TGAGYFMQFS TSSGSAEEAA LLESRILYPK RKQQCLQFFY KMTGSPSDRL VVWVRRDDST GNVRKLVKVQ TFQGDDDHNW KIAHVVLKEE QKFRYLFQGT KGDPQNSTGG IYLDDITLTE TPCPTGVWTV RNFSQVLENT SKGDKLQSPR FYNSEGYGFG VTLYPNSRES SGYLRLAFHV CSGENDAILE WPVENRQVII TILDQEPDVR NRMSSSMVFT TSKSHTSPAI NDTVIWDRPS RVGTYHTDCN CFRSIDLGWS GFISHQMLKR RSFLKNDDLI IFVDFEDITH LSQHHHHHH