Recombinante muis BTLA eiwit

BTLA, also known as B- and T-lymphocyte attenuator isoform 1, is an inhibitory molecule which belongs to the Ig superfamily. It is a type 1 transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. This protein is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1) . Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers. It is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands. And, it is a ligand for tumor necrosis factor (receptor) superfamily, member 14 (TNFRSF14), also known as herpes virus entry mediator (HVEM) . BTLA-HVEM complexes negatively regulate T-cell immune responses. Recombinant mouse BTLA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Btla, B- and T-lymphocyte attenuator isoform 1, BTLA, BTLA1, CD272

HEK293

Mouse

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

44.1 kDa (390aa)

ATGP4003-10 µg, ATGP4003-50 µg, ATGP4003-100 µg, ATGP4003-200ug, ATGP4003-250 µg, ATGP4003-500 µg, ATGP4003-1 mg, ATGP4003-10 µg, ATGP4003-20 µg, ATGP4003-50 µg, ATGP4003-100 µg, ATGP4003-250 µg, ATGP4003-500 µg, ATGP4003-1 mg, ATGP4003-10, ATGP4003-20, ATGP4003-50, ATGP4003-100, ATGP4003-250, ATGP4003-500, ATGP4003-1

Watanabe N., et al, (2003) Nat. Immunol. 4:670-679. ; ; Gavrieli M., et al, (2003) Biochem. Biophys. Res. Commun. 312:1236-1243.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

AAI08964.1

Q32MV9

Mouse

Asp

EKATKR NDEECEVQLN IKRNSKHSAW TGELFKIECP VKYCVHRPNV TWCKHNGTIW VPLEVGPQLY TSWEENRSVP VFVLHFKPIH LSDNGSYSCS TNFNSQVINS HSVTIHVRER TQNSSEHPLI ISDIPDATNA SGPSTMEKRP G