Recombinante muis Carboxyl Ester Lipase/CEL-eiwit

CEL, also known as bile salt-activated lipase, is one of the Type-B carboxyl esterase/lipase family of enzymes. It is an enzyme produced by the adult pancreas and aids in the digestion of fats. Also, it catalyzes fat and vitamin absorption and acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. This protein is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract. Recombinant mouse CEL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 100,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umoleof p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Cel, 1810036E18Rik, BAL, BSSL, Carboxyl ester lipase, Cholesterol esterase, Pancreaticlysophospholipase, Sterol esterase, Lip1, Bile salt-activated lipase, Bile salt-stimulated lipase

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 100,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

64.5 kDa (585aa)

Carboxyl Ester Lipase, CEL, ATGP3905-10 µg, ATGP3905-20 µg, ATGP3905-50 µg, ATGP3905-100 µg, ATGP3905-250 µg, ATGP3905-500 µg, ATGP3905-1 mg, ATGP3905-10, ATGP3905-20, ATGP3905-50, ATGP3905-100, ATGP3905-250, ATGP3905-500, ATGP3905-1

Xiao X., et al. (2016) J Biol Chem. 291:23224-23236.; ; Shindo K., et al. (2017) Oncotarget. 8:50824-50831.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_034015

Q64285

Mouse

Ala21