Recombinante muis Carboxylesterase 3/CES3-eiwit

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Carboxylesterase 1D, Ces1d, Ces3, TGH

Baculovirus

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 80,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

60.9 kDa (555aa)

Carboxylesterase3, CES3, Carboxylesterase 1D, Ces1d, Ces3, TGH, ATGP3467-10 µg, ATGP3467-20 µg, ATGP3467-50 µg, ATGP3467-100 µg, ATGP3467-250 µg, ATGP3467-500 µg, ATGP3467-1 mg, ATGP3467-10, ATGP3467-20, ATGP3467-50, ATGP3467-100, ATGP3467-250, ATGP3467-500, ATGP3467-1

Soni KG., et al. (2004) J. Biol. Chem. 279:40683-40689.; ; Sanghani SP., et al. (2004) Drug Metab Dispos. 32:505-511.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_444430

Q8VCT4

Mouse

Tyr19