Recombinante muis GalNac Transferase 1/GALNT1-eiwit

Galnt1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant mouse Galnt1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1, Protein-UDP acetylgalactosaminyltransferase 1, pp-GaNTase 1, Polypeptide N-acetylgalactosaminyltransferase 1, Polypeptide GalNAc transferase 1, Galnt1, GalNAc-T1

Baculovirus

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

60.5 kDa (528aa)

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1, Protein-UDP acetylgalactosaminyltransferase 1, pp-GaNTase 1, Polypeptide N-acetylgalactosaminyltransferase 1, Polypeptide GalNAc transferase 1, Galnt1, GalNAc-T1, ATGP3718-10 µg, ATGP3718-20 µg, ATGP3718-50 µg, ATGP3718-100 µg, ATGP3718-250 µg, ATGP3718-500 µg, ATGP3718-1 mg, ATGP3718-010, ATGP3718-020, ATGP3718-050, ATGP3718-100, ATGP3718-250, ATGP3718-500, ATGP3718-01M

Li C., et al, (2016) Cancer Res. 76:1273-1283.; ; Huang MJ., et al, (2015) Oncotarget. 6:5650-5665

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_038842

O08912

Mouse

Ala

ADPGLPAGDV LELVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSRGQVITF LDAHCECTAG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRLGLRRKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCTGSRSQQ WLLRNVTLPE IFHHHHHH