Recombinante muis LIFR-alfa/LIFR-eiwit

LIFR alpha, also known as CD118, is a member of the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M. The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR alpha has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway. LIFR alpha is down regulated in a number of breast carcinomas and may serve a prognostic tool. Recombinant mouse LIFR alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mouse LIFR alpha inhibits human LIF (Cat# ATGP3533) induced cell proliferation in the TF-1 human erythroleukemic cells. The ED50 range is ≤ 1 µg/mL.

Leukemia inhibitory factor receptor, LIF receptor, LIF-R, LIF receptor subunit alpha, D-factor, CD118

Baculovirus

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 µg/mL with Mouse LIF.

89.6kDa (794aa)

, ATGP3958-10 µg, ATGP3958-20 µg, ATGP3958-50 µg, ATGP3958-100 µg, ATGP3958-250 µg, ATGP3958-500 µg, ATGP3958-1 mg, ATGP3958-10, ATGP3958-20, ATGP3958-50, ATGP3958-100, ATGP3958-250, ATGP3958-500, ATGP3958-1

Gearing DP., et al (1991) The EMBO Journal. 10: 2839–2848.; ; Layton MJ., et al. (1992) Proc. Natl. Acad. Sci. 89:8616.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_038612

P42703

Mouse

Leu

LKRGVQD LKCTTNNMRV WDCTWPAPLG VSPGTVKDIC IKDRFHSCHP LETTNVKIPA LSPGDHEVTI NYLNGFQSKF TLNEKDVSLI PETPEILDLS ADFFTSSLLL KWNDRGSALP HPSNATWEIK VLQNPRTEPV ALVLLNTMLS GKDTVQHWNW TSDLPLQCAT HSVSIRWHID SPHFSGYKEW SDWSPLKNIS WIRNTETNVF PQDKVVLAGS NMTICCMSPT KVLSGQIGNT LRPLIHLYGQ TVAIHILNIP VSENSGTNII FITDDDVYGT VVFAGYPPDV PQKLSCETHD LKEIICSWNP GRITGLVGPR NTEYTLFESI SGKSAVFHRI EGLTNETYRL GVQMHPGQEI HNFTLTGRNP LGQAQSAVVI NVTERVAPHD PTSLKVKDIN STVVTFSWYL PGNFTKINLL CQIEICKANS KKEVRNATIR GAEDSTYHVA VDKLNPYTAY TFRVRCSSKT FWKWSRWSDE KRHLTTEATP SKGPDTWREW SSDGKNLIVY WKPLPINEAN GKILSYNVSC SLNEETQSVL EIFDPQHRAE IQLSKNDYII SVVARNSAGS SPPSKIASME IPNDDITVEQ AVGLGNRIFL TWRHDPNMTC DYVIKWCNSS RSEPCLLDWR KVPSNSTETV IESDQFQPGV RYNFYLYGCT NQGYQLLRSI IGYVEELAPI VAPNFTVEDT SADSILVKWD DIPVEELRGF LRGYLFYFQK GERDTPKTRS LEPHHSDIKL KNITDISQKT LRIADLQGKT SYHLVLRAYT HGGLGPEKSM FVVTKENS