Recombinante muis RAPSN eiwit

43 kDa receptor-associated protein of the synapse, also known as RAPSN, is expressed in the postsynaptic membrane of skeletal muscle. RAPSN is required for the clustering of nicotinic acetylcholine receptors (nAChR) . It self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs) . RAPSN interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. This protein modifies trafficking of AChR within the cell. Overexpression inhibits agrin-induced AChR clustering pathway. Recombinant human RAPSN protein, fused to His-tag at N-terminus, was expressed in E. coli.

43 kDa receptor-associated protein of the synapse, 43kDa, Nraps, Raps, rapsyn

E.coli

Mouse

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

48.8 kDa (435aa)

RAPSN, 43 kDa receptor-associated protein of the synapse, 43kDa, Nraps, Raps, rapsyn, ATGP2405-10 µg, ATGP2405-20 µg, ATGP2405-50 µg, ATGP2405-100 µg, ATGP2405-250 µg, ATGP2405-500 µg, ATGP2405-1 mg, ATGP2405-10, ATGP2405-20, ATGP2405-50, ATGP2405-100, ATGP2405-250, ATGP2405-500, ATGP2405-1

Maimone M M., et al. (1999) Mol Cell Neurosci. 14:340-354.; ; Han H., et al. (1999) J Neurocytol. 28:763-775;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Neuroscience

NP_033049

P12672

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH LRCLQNNGTR SCPNCRRSSM KPGFV