Recombinante muis SEPSECS-eiwit

Sepsecs, also known as O-phosphoseryl-tRNA (Sec) selenium transferase, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant mouse Sepsecs protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein

E.coli

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

57.7 kDa (527aa) confirmed by MALDI-TOF

O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein, O-phosphoseryl-tRNA (Sec) selenium transferase, AA986712, D5Ertd135e, SecS, SLA, Sepsecs, O phosphoseryl tRNA (Sec) selenium transferase, Sep tRNA : Sec tRNA synthase, ATGP3813-10 µg, ATGP3813-20 µg, ATGP3813-50 µg, ATGP3813-100 µg, ATGP3813-250 µg, ATGP3813-500 µg, ATGP3813-1 mg, ATGP3813-010, ATGP3813-020, ATGP3813-050, ATGP3813-100, ATGP3813-250, ATGP3813-500, ATGP3813-01M

Palioura S., et al. (2009) Science. 325 (5938) :321-5.; ; Yuan J., et al. (2006) Proc. Natl Acad Sci U.S.A. 103 (50) :18923-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.2M NaCl, 50% glycerol

Enzymes & Proteases

NP_766078

Q6P6M7

Mouse

MGSSHHHHHH SSGLVPRGSH MGSMNPESFA AGERRVSPAY VRQGCEARRA HEHLIRLLLE QGKCPEDGWD ESTLELFLHE LAVMDSNNFL GNCGVGEREG RVASALVARR HYRFIHGIGR SGDISAVQPK AAGSSLLNKI TNSLVLNVIK LAGVHSVASC FVVPMATGMS LTLCFLTLRH KRPKAKYIIW PRIDQKSCFK SMVTAGFEPV VIENVLEGDE LRTDLKAVEA KIQELGPEHI LCLHSTTACF APRVPDRLEE LAVICANYDI PHVVNNAYGL QSSKCMHLIQ QGARVGRIDA FVQSLDKNFM VPVGGAIIAG FNEPFIQDIS KMYPGRASAS PSLDVLITLL SLGCSGYRKL LKERKEMFVY LSTQLKKLAE AHNERLLQTP HNPISLAMTL KTIDGHHDKA VTQLGSMLFT RQVSGARAVP LGNVQTVSGH TFRGFMSHAD NYPCAYLNAA AAIGMKMQDV DLFIKRLDKC LNIVRKEQTR ASVVSGADRN KAEDADIEEM ALKLDDVLGD VGQGPAL