Recombinante muis SOST/Sclerostin-eiwit

SOST, also known as Sclerostin, is a member of the cerberus/DAN family. This protein is produced primarily by the osteocyte but is also expressed in other tissues, and has anti-anabolic effects on bone formation. It was originally believed to be a nonclassical bone morphogenetic protein (BMP) antagonist. More recently, sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signaling pathway. The inhibition of the Wnt pathway leads to decreased bone formation. Although the underlying mechanisms are unclear, it is believed that the antagonism of BMP-induced bone formation by sclerostin is mediated by Wnt signaling, but not BMP signaling pathways. Mutations in the gene that encodes the sclerostin protein are associated with disorders associated with high bone mass, sclerosteosis and van Buchem disease. Recombinant mouse SOST/Sclerostin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

SOST, Sclerostin, 5430411E23Rik

HEK293

Mouse

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS – PAGE

21.9kDa (194aa)

, ATGP3975-10 µg, ATGP3975-20 µg, ATGP3975-50 µg, ATGP3975-100 µg, ATGP3975-250 µg, ATGP3975-500 µg, ATGP3975-1 mg, ATGP3975-10, ATGP3975-20, ATGP3975-50, ATGP3975-100, ATGP3975-250, ATGP3975-500, ATGP3975-1

Winkler DG, et al. (2003) The EMBO Journal. 22:6267–6276.; ; Li X, et al. (2005) The Journal of Biological Chemistry. 280:19883–19887.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_077769.4

Q99P68

Mouse

Gln 24