Recombinante muis Thioredoxin-1-eiwit

Thioredoxin-1 is a low molecular weight redox protein. Thioredoxin-1 contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Thioredoxin-1 also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant Mouse Thioredoxin-1 was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >60 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin

TRX1, TRX2, Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, Thioredoxin-1, ADF, Surface associated sulphydryl protein, TXN protein, ATL derived factor, DKFZp686B1993, MGC61975, SASP, Thioredoxin, TRDX, TRX, TRX 1, TXN

E.coli

Mouse

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is >60 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin

14.1 kDa (128aa) confirmed by MALDI-TOF

Thioredoxin1, ATGP3867-10 µg, ATGP3867-20 µg, ATGP3867-50 µg, ATGP3867-100 µg, ATGP3867-250 µg, ATGP3867-500 µg, ATGP3867-1 mg, ATGP3867-10, ATGP3867-20, ATGP3867-50, ATGP3867-100, ATGP3867-250, ATGP3867-500, ATGP3867-1

Pigiet VP., et al. (1986) Proc. Natl.Acad.Sci. uSA 83 (20) :7643-7.; ; Lundstrom J., et al. (1990) J.Biol.Chem. 265 (16) :9114-20.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Homeostasis

NP_035790

P10639

Mouse

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