Recombinante muis TRANCE/RANKL/TNFSF11-eiwit

Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse Tnfsf11 protein was expressed in E. coli and purified by using conventional chromatography.

Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/mL.

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

E.coli

Mouse

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/mL.

17.9 kDa (160aa) confirmed by MALDI-TOF

TRANCE, RANK L, TNFSF11, ATGP3915-10 µg, ATGP3915-20 µg, ATGP3915-50 µg, ATGP3915-100 µg, ATGP3915-250 µg, ATGP3915-500 µg, ATGP3915-1 mg, ATGP3915-10, ATGP3915-20, ATGP3915-50, ATGP3915-100, ATGP3915-250, ATGP3915-500, ATGP3915-1

Lam J., et al. (2001) . J. Clin. Invest. 108 (7) :971-9.; ; Ito S., et al. (2002) . J. Biol. Chem. 277 (8) :6631-6.

Park KH, et al. Zinc inhibits osteoclast differentiation by suppression of Ca2+-Calcineurin-NFATc1 signaling pathway. (Cell Commun Signal. 2013) {https://pubmed.ncbi.nlm.nih.gov/24088289/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid In. 20 mM Tris-HCl buffer (pH8.5) containing 0.1M NaCl

Hormones & Cytokines

NP_035743

O35235

Mouse

MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID