Recombinante rat Agrin-eiwit

Agrin, also known as AGRN, is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis The agrin gene is expressed in rat embryonic nervous system and muscle. This protein is concentrated at synapses, where it may play a role in development and regeneration. When secreted, agrin binds to several receptors on the surface of skeletal muscle. Recombinant Rat Agrin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

AGRN, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, C90, Agrin C-terminal 22 kDa fragment, C22, AGR

Baculovirus

Rat

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

82.5 kDa (766aa)

Agrin, ATGP3929-10 µg, ATGP3929-20 µg, ATGP3929-50 µg, ATGP3929-100 µg, ATGP3929-250 µg, ATGP3929-500 µg, ATGP3929-1 mg, ATGP3929-10, ATGP3929-20, ATGP3929-50, ATGP3929-100, ATGP3929-250, ATGP3929-500, ATGP3929-1

Neuron., et al, (1991) Rupp F. 6 (5) :811-823.; ; Cell., et al, (1996) Glass DJ. 17;85 (4) :513-523.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_786930

P25304

Rat

Ala

SCYNSPL GCCSDGKTPS LDSEGSNCPA TKAFQGVLEL EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFWSVR LRELGPGKLV RAIVDVHFDP TTAFQASDVG QALLRQIQVS RPWALAVRRP LQEHVRFLDF DWFPTFFTGA ATGTTAAMAT ARATTVSRLP ASSVTPRVYP SHTSRPVGRT TAPPTTRRPP TTATNMDRPR TPGHQQPSKS CDSQPCLHGG TCQDQDSGKG FTCSCTAGRG GSVCEKVQPP SMPAFKGHSF LAFPTLRAYH TLRLALEFRA LETEGLLLYN GNARGKDFLA LALLDGRVQF RFDTGSGPAV LTSLVPVEPG RWHRLELSRH WRQGTLSVDG ETPVVGESPS GTDGLNLDTN LYVGGIPEEQ VAMVLDRTSV GVGLKGCIRM LDINNQQLEL SDWQRAAVQS SGVGECGDHP CLPNPCHGGA LCQALEAGMF LCQCPPGRFG PTCADEKSPC QPNPCHGAAP CRVLSSGGAK CECPLGRSGT FCQTVLETAG SRPFLADFNG FSYLELKGLH TFERDLGEKM ALEMVFLARG PSGLLLYNGQ KTDGKGDFVS LALHNRHLEF CYDLGKGAAV IRSKEPIALG TWVRVFLERN GRKGALQVGD GPRVLGESPK SRKVPHTMLN LKEPLYIGGA PDFSKLARGA AVSSGFSGVI QLVSLRGHQL LTQEHVLRAV DVSPFADHPC TQALGNPCLN GGSCVPREAT YECLCPGGFS GLHCEKGLVE